Conferencias, 9no. Encuentro

Por defecto: 
PARTIAL IMMOBILIZATION MEDIATED BY LOW DENSITY POLYETHYLENE NANOPARTICLES ON C. ELEGANS
Diana Stephanie Maving Sanchez

Última modificación: 2023-10-06

Resumen


Micro and nanoplastics are ubiquitous in the environment and
there is ample evidence of their adverse effects on aquatic
ecosystems. However, there is a dearth of reports on its effects on
terrestrial ecosystems. In this study we characterized polyethylene
(PE) (254.0nm–932.0nm) particles with Dynamic light scattering and
optical photothermal infrared and Raman spectroscopy and evaluated its
effects on the nematode Caenorhabditis elegans (C. elegans). C.
elegans was exposed to suspensions of bacteria without (control) or
with PE at concentrations of (100 mg L -1 and 190 mg L -1) on agar
plates for 4 days. Using movement decline as a measure of health, we
used Wormlab software to evaluate the effect of PE nanoparticles on
the average speed of C. elegans. In addition, dark field hyperspectral
microscopy was used to corroborate the ingestion of PE by the
nematodes. We believe that with these applied techniques we could
obtain the detection and distribution of PE nanoparticles inside C.
elegans as well as study their toxicity to get a comprehensive
understanding of how it affects their mobility.